The chemicals used were sodium hydroxide and Rhodamine Water Tracer.
IDENTIFICATION AND USE: Rhodamine WT a fluorescent, xanthene dye, has long been used as a hydrologic tracer in surface water systems. HUMAN STUDIES: There are no data available. ANIMAL STUDIES: Rhodamine WT is reported to be a skin and eye irritant. Mammalian toxicity data available indicated a low to moderate acute injection toxicity in the mouse. Rhodamine WT produced mutations in the Salmonella typhimurium (Ames test), and was a weak inducer of chromosomal effects and DNA damage in cultured mammalian cells. No chromosomal damage or sperm abnormalities were seen in mice treated by intraperitoneal injection. ECOTOXICITY STUDIES: There was no aquatic toxicity detected for Rhodamine WT in oyster eggs, silver salmon and Donaldson trout.
1.2 Skin, Eye, and Respiratory Irritations
Rhodamine WT is reported to be a skin and eye irritant.

